UV‑Vis Spectrophotometer
A UV‑Vis Spectrophotometer is an advanced analytical device
used to measure how substances absorb ultraviolet and visible light. UV‑Vis delivers high precision, rapid, and
reproducible results, making it indispensable for quality control, purity
verification, and research in many disciplines.
The instrument works by passing light of specific
wavelengths (in the ultraviolet and visible spectrum) through a sample. As
different compounds absorb light differently, it measures the amount of light
absorbed at each wavelength. The absorption data then allows quantification and
identification of chemical species, even when present in very low
concentrations, using laws like Beer‑Lambert.
A UV‑Vis Spectrophotometer can analyze a wide variety of
materials and applications, including:
- Active
pharmaceutical ingredients (APIs) and excipients
- Vitamins,
nutraceuticals, food colorants, preservatives
- Biological
molecules like proteins, nucleic acids, enzymes
- Environmental
analytes (pollutants, heavy metals)
- Quality
control of dyes, inks, and industrial chemicals
- Monitoring
reaction kinetics and stability studies
- Natural
compounds and plant extracts
