Treated tissue culture (T.C.) flasks served as fundamental tools for the in vitro cultivation of adherent cells. These sterile polystyrene vessels possessed surfaces that were modified to enhance cellular attachment and proliferation. Typically, a gas plasma treatment was employed to render the flask surface more hydrophilic and negatively charged, thereby promoting the adhesion and spreading of cells. This facilitated the establishment of a monolayer culture, mimicking the in vivo environment required by many cell types for growth
Test Details
Procedures
- Adherent cells were seeded into a sterile treated T.C. flask containing appropriate culture medium and incubated under standard conditions to allow for attachment and growth.
- If the assay involved a treatment, it was administered to the cells in the flask. Following treatment or for control conditions, the cells were further incubated for a specified duration.
- At the experimental endpoint, cells were detached from the flask surface and processed for the desired analysis, such as cell counting, viability assessment, or downstream molecular studies.
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