Description:
The polymerase chain reaction (PCR) is a test tube system for DNA replication which allows a “target” DNA sequence to be selectively amplified several million-fold in just a few hours.
Test Details
Methods/ procedure:
- A PCR tube is prepared where a PCR master mix (dNTPs, Taqpol, buffer, sterile water) is mixed with the DNA template in appropriate amounts.
- Polymerase chain reaction is carried out according to customized programs of the following main steps:
- Denaturing step (95C): DNA strands separate
- Annealing step: primers anneal to the separated strands. Temperature varies according to the primers’ melting temperature (Tm)
- Extension step (72C): new DNA strands are built through primer extension by Taq polymerase
- Repeat for n cycles
Related Devices
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Thermocyler
a laboratory instrument used to amplify DNA segments through a process called the polymerase chain reaction (PCR)
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