The MIC (Minimum Inhibitory Concentration) defines the lowest antimicrobial concentration that prevents visible microbial growth, while the MBC/MFC (Minimum Bactericidal/Fungicidal Concentration) identifies the lowest concentration required to kill ≥99.9% of the initial inoculum.
Test Details
Procedure
Products are serially diluted 1:2 in broth (usually MHB) up to 1:32,768, then mixed with a standardized microbial suspension and incubated for 18–24 hours to determine the MIC—the lowest concentration that visibly inhibits growth. For MBC, samples from the MIC and preceding dilutions are plated and incubated to identify the lowest concentration yielding no colony growth.Application
- Antibiotic development and resistance profiling
- Comparison of generics vs. reference drugs
- Preservative efficacy validation
Reference
The test is performed in accordance with USP/FDAContact Us
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