A killing Time Assay is a test that measures the ability of an antimicrobial agent (e.g., antibiotic, disinfectant, chemical) to kill or reduce the number of viable microorganisms.
Test Details
Procedure
Prepare a standardized suspension of the test microorganism and expose it to the antimicrobial agent at a specified concentration and time. After incubation, neutralize the antimicrobial agent if needed, then plate samples on agar to determine the number of surviving colony-forming units (CFU).Finally, calculate the reduction in viable microorganisms compared to a control without the antimicrobial agent.
Application
- Evaluating antimicrobial potency of new compounds.
- Testing sterilization and sanitation effectiveness in industry.
Reference
Jorgensen, J. H., & Pfaller, M. A. (2015). Manual of Clinical Microbiology (11th ed.).Contact Us
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