Insertion of target gene(s) into plasmid vectors and propagation in appropriate hosts (bacterial [E. coli], mammalian cell line, etc.)
Test Details
- Choice of host organism and cloning vector
- Preparation of insert DNA
- Preparation for insertion
- Creation of recombinant DNA
- Introduction of recombinant DNA into host organism
- Selection of organisms containing recombinant DNA
- Screening for clones with desired DNA inserts and biological properties.
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