Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR)

The polymerase chain reaction (PCR) is a test tube system for DNA replication which allows a “target” DNA sequence to be selectively amplified several million-fold in just a few hours.

Test Details

Methods/ procedure:
  1. A PCR tube is prepared where a PCR master mix (dNTPs, Taqpol, buffer, sterile water) is mixed with the DNA template in appropriate amounts.
  2. Polymerase chain reaction is carried out according to customized programs of the following main steps:
    1. Denaturing step (95C): DNA strands separate
    2. Annealing step: primers anneal to the separated strands. Temperature varies according to the primers’ melting temperature (Tm)
  • Extension step (72C): new DNA strands are built through primer extension by Taq polymerase
  1. Repeat for n cycles

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