DNA cloning (Recombinant plasmid & Transformation)

DNA cloning (Recombinant plasmid & Transformation)

Insertion of target gene(s) into plasmid vectors and propagation in appropriate hosts (bacterial [E. coli], mammalian cell line)

Test Details

  1. Choice of host organism and cloning vector
  2. Preparation of insert DNA
  3. Preparation for insertion
  4. Creation of recombinant DNA
  5. Introduction of recombinant DNA into host organism
  6. Selection of organisms containing recombinant DNA
  7. Screening for clones with desired DNA inserts and biological properties.

Devices that perform this test

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