Agarose Gel Electrophoresis

Agarose Gel Electrophoresis

Description:

is a common laboratory technique that uses a porous gel and an electric field to separate macromolecules like DNA, RNA, and proteins by size and charge. Negatively charged molecules, such as DNA, move through the gel toward the positive electrode, with smaller fragments moving faster than larger ones. After separation, the molecules are visualized using a stain that causes them to appear as distinct bands, allowing to determine their sizes by comparing them to a known molecular weight marker. Gel can be prepared at concentration of 0.1% to 3%.

Test Details

Applications:  DNA & RNA Analysis
  • DNA Fingerprinting:
Separating DNA fragments for forensic analysis (e.g., at crime scenes) and paternity testing.
  • PCR and Cloning:
Confirming DNA amplification in Polymerase Chain Reaction, assessing ligation efficiency, and screening colonies in molecular cloning.
  • Genetic Analysis:
Detecting genetic variants and analyzing genes associated with diseases.
  • Taxonomy:
Distinguishing between different species for taxonomic studies by analyzing their genetic differences.
  • RNA Analysis:
Evaluating the success of in vitro transcription by running newly synthesized RNA on a gel.   Protein Analysis 
  • Protein Profiling:
Studying the structure and function of proteins and detecting abnormal proteins in biological fluids like serum.
  • Clinical Diagnostics:
Diagnosing conditions like hemoglobinopathies and monitoring lipoproteins and serum protein phenotypes.
  • Vaccine Testing:
Assessing the purity and concentration of vaccines and analyzing different types and levels of antibodies.
  • Other Applications
Antibiotic Resistance Analysis: Studying DNA mobility shifts to analyze the genetic basis of antibiotic resistance in bacteria.
  • Product Authenticity:
Verifying the authenticity of certain products, such as identifying the presence of salmon DNA in products claimed to be salmon.
  • Evolutionary Studies:
Analyzing genetic similarity among populations or species to understand evolutionary relationships.

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