RT-PCR (Reverse Transcription PCR), it’s used to amplify DNA after reverse transcription of RNA, enabling the study of gene expression.
Technical Specifications
RT-PCR is used in various applications, including:
- Gene expression analysis: Quantifying the amount of specific RNA transcripts in a sample.
- Detection of viral RNA: Identifying and quantifying viral RNA in clinical samples.
- Generating cDNA for cloning and sequencing: Preparing DNA templates for further molecular biology experiments.