Genotoxicity assays are crucial tests performed in cell culture laboratories to evaluate the potential of chemical, physical, or biological agents to cause damage to genetic material (DNA). This damage can lead to mutations, chromosomal abnormalities, and ultimately, diseases like cancer.
Test Details
Procedure
- Cells were selected and grown in sterile conditions with specific nutrients, temperature, and CO2. Cell health and growth stage were monitored.
- The test agent was prepared in a range of concentrations. Cells were exposed for a specific time, and sometimes, metabolic activation systems were added. Controls were used for comparison.
- Analysis: This varied by assay:
- Micronucleus Assay: Cells were treated, stained, and examined for micronuclei.
- Chromosomal Aberration Assay: Metaphase cells were stained and examined for structural changes.
- Comet Assay: Cells were lysed, and DNA fragments were electrophoresed; tail length indicated damage.
- HPRT/TK Mutation Assays: Cells were cultured in selective medium to identify mutants.
Related Devices
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CO2 Cell Culture Incubator
A carbon dioxide (CO2) incubator is a specialized laboratory equipment used for cell culture, which helps to maintain optimal & precise conditions of temperature, humidity, and carbon dioxide levels required…
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