Cytotoxicity assays are fundamental tools in pharmaceutical research. They serve various critical purposes, such as identifying potential anticancer drugs that inhibit cell growth or cause cell death, determining the concentration at which a substance inhibits cell proliferation by 50% (IC50 values) for a range of compounds (drugs, extracts, formulations), evaluating the impact of biological molecules (growth factors, cytokines, mitogens, nutrients) on cell growth, and studying physiological factors that suppress cellular proliferation
Test Details
Procedures
- Cells were grown in plates and then exposed to various concentrations of a test compound, with control cells left untreated.
- MTT solution was added, allowing viable cells to convert it into formazan crystals, which were subsequently dissolved.
- absorbance of the resulting solution was measured to quantify viable cells in treated samples relative to the control, indicating cytotoxicity.
- Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1-2), 55-63.
- Riss, T. L., Moravec, R. A., Niles, A. L., Duellman, S., Benink, H. A., Worzella, T. J., & Minor, L. (2013). Cell Viability Assays. In Assay Guidance Manual. Eli Lilly & Company and the National Center for Advancing Translational Sciences
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